Two antimicrobial genes from Aegilops tauschii Cosson identified by the Bacillus subtilis expression system
نویسندگان
چکیده
منابع مشابه
Essential Bacillus subtilis genes.
To estimate the minimal gene set required to sustain bacterial life in nutritious conditions, we carried out a systematic inactivation of Bacillus subtilis genes. Among approximately 4,100 genes of the organism, only 192 were shown to be indispensable by this or previous work. Another 79 genes were predicted to be essential. The vast majority of essential genes were categorized in relatively fe...
متن کاملAbh and AbrB control of Bacillus subtilis antimicrobial gene expression.
The Bacillus subtilis abh gene encodes a protein whose N-terminal domain has 74% identity to the DNA-binding domain of the global regulatory protein AbrB. Strains with a mutation in abh showed alterations in the production of antimicrobial compounds directed against some other Bacillus species and gram-positive microbes. Relative to its wild-type parental strain, the abh mutant was found defici...
متن کاملIdentification of two myo-inositol transporter genes of Bacillus subtilis.
Among hundreds of mutants constructed systematically by the Japanese groups participating in the functional analysis of the Bacillus subtilis genome project, we found that a mutant with inactivation of iolT (ydjK) exhibited a growth defect on myo-inositol as the sole carbon source. The putative product of iolT exhibits significant similarity with many bacterial sugar transporters in the databas...
متن کاملTwo genes encoding uracil phosphoribosyltransferase are present in Bacillus subtilis.
Uracil phosphoribosyltransferase (UPRTase) catalyzes the key reaction in the salvage of uracil in many microorganisms. Surprisingly, two genes encoding UPRTase activity were cloned from Bacillus subtilis by complementation of an Escherichia coli mutant. The genes were sequenced, and the putative amino acid sequences were deduced. One gene showed a high level of homology to UPRTases from other o...
متن کاملexpression profiling of genes involved in signaling process in aegilops tauschii under salinity stress
salinization of crop lands is one of the most important reasons for reduction in productivity and threats food security. activation of signaling process in plants is the first step during sense environmental cues and stress signals. identification of the components of plant intracellular signaling mechanisms, pathways, and networks in response to salt stress in aegilops tauschii (the d genome d...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Scientific Reports
سال: 2020
ISSN: 2045-2322
DOI: 10.1038/s41598-020-70314-5